Comparision of iron-regulated outer membrane proteins (IrOMP) and iron-sufficient outer membrane proteins (IsOMP) of Pasteurella multocida strains of porcine origin

Immunogenicity of iron-regulated outer membrane proteins of Pasteurella multocida B:2 in mice model.

The present study was conducted to investigate the role of iron-regulated outer membrane proteins (IROMP) of Pasteurella multocida B:2 in mice as potential immunogens. Outer membrane proteins extracted from P. multocida B:2 grown under normal (OMP) and iron-deficient (IROMP) conditions were subjected to discontinuous SDS-PAGE. Nine polypeptides of MW ranging from 85.1 to 16.7 kDa from OMP prepa...

A Chimeric Vaccine Consisting of Highly Immunogenic Regions form Escherichia coli Iron Regulated Outer-Membrane Proteins: An In Silico Approach

Identification of Pasteurella multocida CHAPS-soluble outer membrane proteins.

Fowl cholera continues to be of concern to the poultry industry, especially for turkey growers. This disease costs the turkey industry millions of dollars annually. In order to develop improved live attenuated vaccines or subunit vaccines, the outer-membrane proteins of Pasteurella multocida were examined with the use of proteomics. Of the 11 proteins total present in an outer-membrane subfract...

Electrophoretic Profiles of the Outer Membrane Proteins of Pasteurella Multocida Strains Isolated from Cattle

Forty Pasteurella multocida strains of serotype B:2,5 were collected from cattle affected with haemorrhagic septicaemia, and 72 strains mainly of serotype A:3, were isolated from calves with changes typical of bronchopneumonia. The outer membrane proteins (OMPs) were prepared by extraction, with 1% sarcosyl from field strains and two reference strains. OMPs were separated electrophoretically at...

Evaluation of Immunogenicity of Outer Membrane Proteins of Pasteurella Multocida Serotype B:2,5 in Cattle

The studies involved P. multocida of serotype B:2,5 isolated from animal affected with haemorrhagic septicaemia. Outer membrane proteins (OMPs) and iron-regulated outer membrane proteins (IROMPs) were prepared by extraction with 1% sarcosyl from the strain cultured on BHI medium and on a medium supplemented with 2,2’-dipyridyl. The antigen complexes were used to immunize calves. Each vaccine do...